ins2 akita mice attenuates

attenuates retinal inflammation and functional deficits in early …

 · PDF 檔案The Ins2 Akita/+ mice carry a spontaneous mutation (single base-pair substitution) in the insulin 2 (Ins2) gene, resulting in proinsulin protein misfolding and subse-quent beta cell failure, eventually leading to spontaneous insulin- dependent diabetes. 17 The male

Global Transcriptional Programs in Peripheral Nerve Endoneurium …

 · PDF 檔案in Ins2Akita/+ mice We have selected Ins2Akita/+ mice as a model of type 1 DPN. In this mouse model, diabetes is a consequence of an autosomal dominant mutation in the Ins2 gene resulting in the improper folding of the Proinsulin 2 protein leading to Akita/+

Influence of genetic background on albuminuria and …

Previous studies have shown that Akita mice bearing the Ins2(+/C96Y) mutation have significant advantages as a type I diabetes platform for developing models of diabetic nephropathy (DN; Gurley SB, Clare SE, Snow KP, Hu A, Meyer TW, Coffman TM. Am J

Ins2-(C96Y)小鼠模型

南模生物自主研發Ins2-(C96Y)小鼠模型 南模生物利用CRISPR技術自主研發Ins2-(C96Y)小鼠模型（基因型與Akita一致）。從4周齡開始，且癥狀隨著年齡

VISUAL ACUITY AND CONTRAST SENSITIVITY
 · PDF 檔案Ins2 Akita/+ diabetic mice after 10 to 26 weeks of diabetes compared to controls (p < 0.01). Contrast sensitivity was also reduced; however, a significant loss of 16% – 26% was found after 18 weeks of diabetes and continued to decrease up to 26 weeks.

Antagonising Wnt/β-catenin signalling ameliorates lens …

 · Male heterozygous Ins2 Akita mice of C57BL/6 J background (C57BL/6-Ins2 Akita /J, Jackson Laboratory, Bar Harbor, ME, USA) and non-diabetic siblings were used. The Ins2 Akita mice develop severe hyperglycaemia (>13.9 mmol/l) by 4 weeks of age [].

Pparg-P465L Mutation Worsens Hyperglycemia in Ins2 …

Pparg-P465L Mutation Worsens Hyperglycemia in Ins2-Akita Female Mice via Adipose-Specific Insulin Resistance and Storage Dysfunction. Item Preview

Role of sigma 1 receptor in retinal degeneration of the …

Purpose: Sigma receptor 1 (Sigma1R), a nonopioid putative molecular chaperone, has neuroprotective properties in retina. This study sought to determine whether delaying administration of (+)-pentazocine, a high-affinity Sigma1R ligand after onset of diabetes in Ins2 Akita/+ diabetic mice would afford retinal neuroprotection and to determine consequences on retinal phenotype in Ins2 Akita

Nutrients
 · Ins2-Akita mice (C57BL/6J-Ins2Akita) and their wildtype (WT) littermates were purchased from the Jackson Laboratory and bred at the Alfred Research Alliance Animal Centre, Melbourne, Australia. Mice were maintained at the Alfred Research Alliance Animal Centre under a 12 …

Frontiers
 · Consistent with this, calcified arteries from Mgp–/– and Ins2 Akita/+ mice express CD31, multipotent markers like SOX2, Nanog and Oct3/4, and Runx2 and Osterix (74, 75). Interestingly, MGP–/– human VECs cultured with osteogenic medium or BMP2 show upregulation of SOX2, Nanog and Oct3/4 prior to that of Runx2 and Osterix, indicating that multipotent marker appear earlier in the …

JCI
 · Ins2 Akita/+ mice (both males and females) were bred with Tet2 +/– (male and female) mice to obtain Ins2 Akita/+ Tet2 +/– compound mutant mice and controls (). Like the Ins2 Akita/+ mutants, the compound mutant mice showed increased blood glucose levels and reduced body weight — 2 typical physiological characteristics of T1DM ( Figure 1E ).

Sustained intraocular VEGF neutralization results in …

Furthermore, anti-VEGF-treated Ins2 Akita mice exhibited impaired electroretinographic responses, characterized by reduced scotopic a- and b-wave and oscillatory potentials. Immunofluorescent staining revealed impairment of photoreceptors, disruptions of synaptic structures and loss of amacrine and retinal ganglion cells in anti-VEGF treated Ins2^Akita mice.

Low-Dose Streptozotocin Induction Protocol (Mouse)

 · PDF 檔案2. Mice should be fasted prior to injection; four hours is usually sufficient. 3. Place one mouse in the Isoflurane drop jar, according to your local IACUC anesthesia standards. 4. Remove mouse when breathing has slowed and animal is anesthetized 5. Inject 6.

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Infl inflammation on phenotype of diabetic retinopathy

 · PDF 檔案Influence of endotoxin-mediated retinal inflammation on phenotype of diabetic retinopathy in Ins2Akita mice Nermina N Vagaja,1,2 Nicolette Binz,2 Samuel McLenachan,3 Elizabeth P Rakoczy,1,2 Paul G McMenamin4 Additional material is published online only.
，體重不增的明顯糖尿病癥狀，Ins2-(C96Y)雜合子小鼠（以下簡稱Ins2-(C96Y)小鼠）表現出多食，高血糖，多飲